9/9/2023 0 Comments Biorad semi dry blotter![]() Try adding another pad or replace any pads that have lost their resiliency with fresh ones.Ī good indication of over-compression is if the gel has been excessively flattened. The gel/membrane assembly should be held securely between the two halves of the blot module. Also, the pads need to be fully saturated (push down with gloved hand when they are placed in transfer buffer to make sure there are no air bubbles.) It is helpful to use a disposable pipette to place some extra transfer buffer on the surface of each layer as the sandwich is being made. ![]() To ensure that this happens, make sure that you roll over the surface of each layer of the gel/membrane sandwich with a glass pipette to ensure good contact between the gel and the membrane. The gel should be attached to the membrane through capillary action. Poor contact between the gel and the membrane Make sure that the methanol concentration in the transfer buffer is not more than 10–20% and that high-quality, analytical grade methanol is used. It may cause a reduction in pore size, precipitation of some proteins, and some basic proteins to become positively charged or neutral. ![]()
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